Journal:

Article Title: Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei

doi: 10.1128/JB.184.14.4003-4017.2002

Figure Lengend Snippet: Plasmids used in this study

Article Snippet: Plasmids used in this study table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Bacterium Relevant information Plaque formation a Burkholderia mallei NCTC 120 LPS O-antigen mutant; wbiE ::IS 407 A − NCTC 10248 + NCTC 10229 + NCTC 10260 + NCTC 10247 + NCTC 3708 + NCTC 3709 + ATCC 23344 + ATCC 10399 + ATCC 15310 + {"type":"entrez-nucleotide","attrs":{"text":"DB110795","term_id":"83524048","term_text":"DB110795"}} DB110795 Laboratory-passaged ATCC 15310; LPS O-antigen mutant; wbiG ::IS 407 A − BML10 ATCC 23344 (φE125) − DD3008 ATCC 23344::pGSV3008; capsule mutant + Burkholderia pseudomallei 50 strains − Burkholderia thailandensis 32 strains − Burkholderia cepacia Genomovar I; 1 strain − Burkholderia multivorans 2 strains − Burkholderia cepacia Genomovar III; 2 strains − Burkholderia stabilis 1 strain − Burkholderia vietnamiensis 2 strains − Burkholderia gladioli 4 strains − Burkholderia uboniae 1 strain − Burkholderia cocovenans 1 strain − Burkholderia pyrrocinia 1 strain − Burkholderia glathei 1 strain − Burkholderia caryophylli 1 strain − Burkholderia andropogonis 1 strain − Burkholderia kururiensis 1 strain − Burkholderia sacchari 1 strain − Burkholderia spp.

Techniques: Clone Assay, Plasmid Preparation, Selection, TA Cloning

Journal:

Article Title: Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei

doi: 10.1128/JB.184.14.4003-4017.2002

Figure Lengend Snippet: Bacteria used to examine the host range of bacteriophage φE125

Article Snippet: Plasmids used in this study table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Bacterium Relevant information Plaque formation a Burkholderia mallei NCTC 120 LPS O-antigen mutant; wbiE ::IS 407 A − NCTC 10248 + NCTC 10229 + NCTC 10260 + NCTC 10247 + NCTC 3708 + NCTC 3709 + ATCC 23344 + ATCC 10399 + ATCC 15310 + {"type":"entrez-nucleotide","attrs":{"text":"DB110795","term_id":"83524048","term_text":"DB110795"}} DB110795 Laboratory-passaged ATCC 15310; LPS O-antigen mutant; wbiG ::IS 407 A − BML10 ATCC 23344 (φE125) − DD3008 ATCC 23344::pGSV3008; capsule mutant + Burkholderia pseudomallei 50 strains − Burkholderia thailandensis 32 strains − Burkholderia cepacia Genomovar I; 1 strain − Burkholderia multivorans 2 strains − Burkholderia cepacia Genomovar III; 2 strains − Burkholderia stabilis 1 strain − Burkholderia vietnamiensis 2 strains − Burkholderia gladioli 4 strains − Burkholderia uboniae 1 strain − Burkholderia cocovenans 1 strain − Burkholderia pyrrocinia 1 strain − Burkholderia glathei 1 strain − Burkholderia caryophylli 1 strain − Burkholderia andropogonis 1 strain − Burkholderia kururiensis 1 strain − Burkholderia sacchari 1 strain − Burkholderia spp.

Techniques: Mutagenesis

Journal:

Article Title: Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei

doi: 10.1128/JB.184.14.4003-4017.2002

Figure Lengend Snippet: Bacteriophage φE125 integrates into the proline tRNA (UGG) gene in B. mallei and B. thailandensis. (A) The nucleotide sequence of the proline tRNA (UGG) gene of B. mallei ATCC 23344 and B. thailandensis E125. The underlined sequence represents the 49-bp attachment site that is identical in the φE125 genome (attP), the B. mallei chromosome (attB), and the B. thailandensis chromosome (attB). The location of the anticodon in the proline tRNA gene is shown in bold. (B) Schematic representation of integration of the φE125 genome into the proline tRNA (UGG) gene of B. mallei and B. thailandensis. The φE125 genome is depicted as a circle, and the approximate locations of gene1, gene18, gene32, gene33, gene34, gene35, gene70, and the cos site are shown. The B. mallei and B. thailandensis chromosomes are represented as a line, and the location and direction of transcription of orfA and orfB are represented by arrows. The 5′ end of the proline tRNA (UGG) gene is shown as a thin white rectangle, and the 3′ end (the attachment site) is shown as a thin black rectangle. Following site-specific recombination (X), the orfA and orfB genes are separated by the integrated φE125 prophage.

Article Snippet: Plasmids used in this study table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Bacterium Relevant information Plaque formation a Burkholderia mallei NCTC 120 LPS O-antigen mutant; wbiE ::IS 407 A − NCTC 10248 + NCTC 10229 + NCTC 10260 + NCTC 10247 + NCTC 3708 + NCTC 3709 + ATCC 23344 + ATCC 10399 + ATCC 15310 + {"type":"entrez-nucleotide","attrs":{"text":"DB110795","term_id":"83524048","term_text":"DB110795"}} DB110795 Laboratory-passaged ATCC 15310; LPS O-antigen mutant; wbiG ::IS 407 A − BML10 ATCC 23344 (φE125) − DD3008 ATCC 23344::pGSV3008; capsule mutant + Burkholderia pseudomallei 50 strains − Burkholderia thailandensis 32 strains − Burkholderia cepacia Genomovar I; 1 strain − Burkholderia multivorans 2 strains − Burkholderia cepacia Genomovar III; 2 strains − Burkholderia stabilis 1 strain − Burkholderia vietnamiensis 2 strains − Burkholderia gladioli 4 strains − Burkholderia uboniae 1 strain − Burkholderia cocovenans 1 strain − Burkholderia pyrrocinia 1 strain − Burkholderia glathei 1 strain − Burkholderia caryophylli 1 strain − Burkholderia andropogonis 1 strain − Burkholderia kururiensis 1 strain − Burkholderia sacchari 1 strain − Burkholderia spp.

Techniques: Sequencing

Journal: JCI Insight

Article Title: Increased de novo ceramide synthesis and accumulation in failing myocardium

doi: 10.1172/jci.insight.82922

Figure Lengend Snippet: (A) Increased levels of total and individual ceramide species in response to SPTLC1 and SPTLC2 overexpression in AC16 cells (n = 4). (B) Increased cellular apoptosis in response to SPTLC1 and -2 overexpression compared with controls. Percentage of apoptotic cells quantified by annexin-V staining (n = 4, 20×). (C) Analysis of cellular oxidative metabolism by Seahorse analysis. Oxygen consumption rates were measured continuously throughout the experimental period starting at baseline and after addition of oligomycin (Oligo, 1 μM) and carbonylcyanide p-trifluoromethoxyphenylhydrazone (FFCP1, 1 μM). Rot, rotenone; anti, antimycin. (D) Averaged data of basal respiration, ATP turnover, H+ leak, and respiratory capacity in AC16 cells in response to SPTLC1 and SPTLC2 overexpression and controls (n = 3). (E) Cellular ceramide levels in AC16 cells in response to myriocin treatment (n = 5–8 per group). Two-tailed Student’s t test was used for 2 group comparisons ,and one-way ANOVA was used for 3 group comparisons (*P < 0.05, **P < 0.01, ***P < 0.001 versus control).

Article Snippet: After blocking, the membranes were incubated overnight at 4°C with the following primary antibodies: SPTLC1 (A303-407A, Bethyl Laboratories Inc.), SPTLC2 (PA5-21142, Thermo Fisher Scientific), SPTLC3 (sc-86226, Santa Cruz Biotechnolgy Inc.), CERS5 (ab73289, Abcam), CERS2 ( {"type":"entrez-protein","attrs":{"text":"A70389","term_id":"7451552","term_text":"pir||A70389"}} A70389 , Sigma-Aldrich), ASM (sc-11352, Santa Cruz Biotechnolgy Inc.), CERS1 (QC18809, Sigma-Aldrich), and GAPDH (3683, Cell Signaling Technology).

Techniques: Over Expression, Staining, Two Tailed Test